Nations underestimate greenhouse gas emissions from wastewater systems by amounts ranging from 19% to 27%, in part caused by a reliance on 2006 IPCC guidance rather than incorporating updates from a 2019 refinement [Nature Climate Change]

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26 Feb 2026 21 min read

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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?

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